School of Veterinary Medicine

金田 剛治

Takeharu Kaneda

基本情報

所属
日本獣医生命科学大学 獣医学部 教授
学位
博士(獣医学)(日本獣医畜産大学大学院)

J-GLOBAL ID
201501004729774245
researchmap会員ID
B000250269

論文

 23
  • Sachiyo Tanaka, Shuji Suzuki, Satoshi Soeta, Takeharu Kaneda, Yasushi Hara
    Open Veterinary Journal 13(12) 1708-1708 2023年  
    Background: We previously reported that myocardial fibrosis may be one of the causes of left ventricular hypertrophy and cardiac dysfunction in dogs with hyperglucocorticism (HGC). The detailed mechanism by which myocardial fibrosis of the left ventricle occurs in dogs with hyperglucocorticism (HGC) remains unclear. Aim: This study investigated the mechanism by which HGC causes fibrosis of the left ventricle.Methods: The impacts of HGC on the heart by comparing samples obtained from high-dose glucocorticoid-treated (P) and untreated (C) dogs. The P group included healthy Beagle dogs (n=6) treated with prednisolone (2 mg/kg, bid, po) for 84 days, and the C group included healthy Beagle dogs (n=6) euthanized for unrelated reasons. In three of the P group dogs, serum was collected before the start of administration (Day 0) and on Day 84 to measure angiotensin II concentrations and oxidative stress markers (8-hydroxy-2’-deoxyguanosine (8OHdG), NADPH oxidase, and superoxide levels). Samples of the left ventricular free wall (LVFW), right ventricular free wall (RVFW), interventricular septum (IVS), and aortic root were harvested from both groups (n = 6 for each group). Using these tissue samples, angiotensin II type 1 receptor (AT1R), 8OHdG, and transforming growth factor β1 (TGFβ1) immunohistochemical stains were performed.Results: The blood NADPH oxidase concentration was significantly higher (P=0.027) in the P group 84 days after initiation of the medication compared to that before prednisolone treatment. By contrast, there was no significant difference in serum angiotensin II (P=0.450), 8OHdG (P=0.068), and superoxide (P=0.057) concentrations. The positive staining rates of AT1R, 8OHdG, and TGFβ1 in the heart (LVFW, RVFW, IVS, and aortic root) were significantly higher in the P group than those in the C group.Conclusion: Angiotensin II and oxidative stress in HGC may cause left ventricular fibrosis in dogs.
  • Hisako Kaneda, Misa Hori, Haruka Shinomiya, Ayaka Nakajima, Shingo Yamazaki, Noriyasu Sasaki, Tsuyoshi Sato, Takeharu Kaneda
    Journal of Food Biochemistry 2022年3月21日  査読有り責任著者
  • 山崎 慎吾, 絹山 寿理亜, 金田 寿子, 佐々木 典康, 金田 剛治
    日本獣医学会学術集会講演要旨集 164回 [JO-22] 2021年9月  
  • Kaneda H, Otomo R, Sasaki N, Omi T, Sato T, Kaneda T
    Journal of pharmacological sciences 140(1) 48-53 2019年5月  査読有り責任著者
  • 稲本 彩, 金田 寿子, 佐々木 典康, 神田 秀憲, 田島 剛, 金田 剛治
    日本獣医学会学術集会講演要旨集 161回 461-461 2018年8月  

MISC

 39

書籍等出版物

 8

講演・口頭発表等

 33

担当経験のある科目(授業)

 3

共同研究・競争的資金等の研究課題

 5