School of Veterinary Medicine

Takeharu Kaneda

  (金田 剛治)

Profile Information

Affiliation
Faculty of Veterinary Science, Nippon Veterinary and Life Science University
Degree
博士(獣医学)(日本獣医畜産大学大学院)

J-GLOBAL ID
201501004729774245
researchmap Member ID
B000250269

Papers

 23
  • Sachiyo Tanaka, Shuji Suzuki, Satoshi Soeta, Takeharu Kaneda, Yasushi Hara
    Open Veterinary Journal, 13(12) 1708-1708, 2023  
    Background: We previously reported that myocardial fibrosis may be one of the causes of left ventricular hypertrophy and cardiac dysfunction in dogs with hyperglucocorticism (HGC). The detailed mechanism by which myocardial fibrosis of the left ventricle occurs in dogs with hyperglucocorticism (HGC) remains unclear. Aim: This study investigated the mechanism by which HGC causes fibrosis of the left ventricle.Methods: The impacts of HGC on the heart by comparing samples obtained from high-dose glucocorticoid-treated (P) and untreated (C) dogs. The P group included healthy Beagle dogs (n=6) treated with prednisolone (2 mg/kg, bid, po) for 84 days, and the C group included healthy Beagle dogs (n=6) euthanized for unrelated reasons. In three of the P group dogs, serum was collected before the start of administration (Day 0) and on Day 84 to measure angiotensin II concentrations and oxidative stress markers (8-hydroxy-2’-deoxyguanosine (8OHdG), NADPH oxidase, and superoxide levels). Samples of the left ventricular free wall (LVFW), right ventricular free wall (RVFW), interventricular septum (IVS), and aortic root were harvested from both groups (n = 6 for each group). Using these tissue samples, angiotensin II type 1 receptor (AT1R), 8OHdG, and transforming growth factor β1 (TGFβ1) immunohistochemical stains were performed.Results: The blood NADPH oxidase concentration was significantly higher (P=0.027) in the P group 84 days after initiation of the medication compared to that before prednisolone treatment. By contrast, there was no significant difference in serum angiotensin II (P=0.450), 8OHdG (P=0.068), and superoxide (P=0.057) concentrations. The positive staining rates of AT1R, 8OHdG, and TGFβ1 in the heart (LVFW, RVFW, IVS, and aortic root) were significantly higher in the P group than those in the C group.Conclusion: Angiotensin II and oxidative stress in HGC may cause left ventricular fibrosis in dogs.
  • Hisako Kaneda, Misa Hori, Haruka Shinomiya, Ayaka Nakajima, Shingo Yamazaki, Noriyasu Sasaki, Tsuyoshi Sato, Takeharu Kaneda
    Journal of Food Biochemistry, Mar 21, 2022  Peer-reviewedCorresponding author
  • 山崎 慎吾, 絹山 寿理亜, 金田 寿子, 佐々木 典康, 金田 剛治
    日本獣医学会学術集会講演要旨集, 164回 [JO-22], Sep, 2021  
  • Kaneda H, Otomo R, Sasaki N, Omi T, Sato T, Kaneda T
    Journal of pharmacological sciences, 140(1) 48-53, May, 2019  Peer-reviewedCorresponding author
  • 稲本 彩, 金田 寿子, 佐々木 典康, 神田 秀憲, 田島 剛, 金田 剛治
    日本獣医学会学術集会講演要旨集, 161回 461-461, Aug, 2018  

Misc.

 39

Books and Other Publications

 8

Presentations

 33

Teaching Experience

 3

Research Projects

 5