井尻 吉信

【方法】M医院（大阪府、阪南市）に通院している2型糖尿病患者のうち、研究の趣旨に同意が得られた患者を無作為に2群に分け、『食事調査結果に対するコメント返却のみ実施した群（以下、対照群）』、『食事調査結果に対するコメント返却と3ヶ月に1度の個別栄養食事指導を実施した群（以下、介入群）』と設定した。研究期間は12ヶ月間とし、各種の身体計測、糖尿病関連の指標、血流依存性血管拡張反応（FMD）ならびに簡易型自記式食事歴法質問票（BDHQ）を用いた食事調査を行った。<br /> 【結果】管理栄養士による”食べる順番療法”を取り入れた継続的な個別栄養食事指導は、体重変化率ならびにHbA1c変化率を有意に低下させた。また、FMD値は各群において有意に増加(血管内皮機能が改善)したが、両群間の比較では有意差は認められなかった。一方、BDHQから得られた栄養素摂取量には差は認められなかった。<br /> 【結論】本法は、無床診療所に通院している2型糖尿病患者の治療効果向

今回調査した地域における無床診療所の管理栄養士配置に対する生活習慣病患者からのニーズは、約7割と高いものであった。今後は無床診療所医師に対する普及活動や、管理栄養士の役割を理解できていない患者に対する啓発活動が必要である。本論文は臨床栄養学領域の業績である。担当部分：全般

抗血栓作用を持つ野菜品種を3ヶ月間、健常者に摂取させた研究。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：全般

ニンニクの血小板反応性抑制作用ならびに内在性血栓溶解能を調べた研究。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：全般

In the quest for prevention of atherothrombotic diseases, an antithrombotic diet may offer a promising approach. The major stumbling block in finding an effective diet is the lack of pathophysiological relevant techniques to detect potential antithrombotic effects of various diet components. Platelet function and coagulation/fibrinolysis tests currently in use do not allow assessment of global thrombotic status and their value in screening diet-components for antithrombotic effects. Recently, we combined the point-of-care shear-induced ex vivo thrombosis test (Global Thrombosis Test-GTT) with the Flow-mediated Vasodilation (FMV) in vivo test and found that the combination improved the assessment of thrombotic status in humans and could be used for screening diet-components for antithrombotic effects. In the present experiments, a combination of GTT, hemostatometry, laser-induced thrombosis tests and FMV were employed for screening. The results show that the overall antithrombotic effect is determined by the effect on thrombus formation and endogenous thrombolytic activities. This study showed a great variation in the observed antithrombotic effect between the tested varieties. Antithrombotic activities were independent from polyphenolic content or antioxidant activities. The presented experimental techniques seem to be suitable for establishing an antithrombotic diet, which may be effective in the prevention of atherothrombotic cardiovascular diseases in humans. © 2016, International Research and Cooperation Association for Bio &amp Socio-Sciences Advancement. All rights reserved.

糖尿病食事療法のうち、栄養基準が大きく変化する糖尿病性腎症第3期Bに該当する患者は、109名中25名であった。このうち、BDHQから得られたたんぱく質摂取量が1.0g/kg標準体重/日を上まっている患者は17名であり、特にこれらの患者は管理栄養士による詳細な栄養指導を受ける必要性が高いことが考えられる。本論文は臨床栄養学領域の業績である。担当部分：全般

わが国の管理栄養士養成教育の在り方について検討した研究。本論文は栄養教育学領域の業績である。担当部分：共同研究につき抽出不可能

わが国の消化器待機的手術ならびに重症患者に対するIED 投与（インパクト®に限定）の現状について調査した研究。本論文は臨床栄養学領域の業績である。担当部分：共同研究につき抽出不可能

抗血栓作用測定法をまとめた総説。本論文は血液生理学領域の業績である。担当部分：共同研究につき抽出不可能

MNA®は高齢者の栄養状態を的確に評価できるだけでなく、生命予後を予測することができるアセスメントツールとして有用とされている。特に、MNA-SF®は4分以内で実施でき、かつ専門的な知識を持たなくても評価が可能であることが特徴である。しかしながら、欧米人のデータを基に開発された手法であるため、日本人高齢者に応用するのは適切であるか問題である。今後は、より多くの日本人高齢者から得られたデータを用いた研究により、さらに信頼性の高いアセスメントツールになることが望まれる。本論文は臨床栄養学領域の業績である。担当部分：全般

病院管理栄養士が「不足していると感じているスキル」や「希望する学び直しプログラム」と、現在各大学で実施されている学び直しプログラムとを比較し、より効果的に個別栄養指導の質を向上させることができる学び直しプログラムを検討することを目的とした。<br /> 複数年の個別栄養指導経験を、短期間プログラムで代替でき、かつ、日頃補いきれない知識を充実させることができるような学び直しプログラムの構築が必要であると考えられた。本論文は臨床栄養学領域の業績である。担当部分：全般

In the present study, we examined the effects of short- and long-term treatment with folic acid (FA) on thrombus formation in vivo in atherogenic mice to explore a novel agent for the prevention of atherothrombotic disease. Apolipoprotein E and low-density lipoprotein receptor double deficient (ApoE-/-LDLR-/-) mice were orally administrated a single bolus of FA (20mg/kg) or fed an atherogenic diet with or without FA (0.02, 0.5, and 1.5mg/kg) for 12 weeks. Thrombus formation and endothelial function were assessed in vivo using the He-Ne laser-induced carotid artery thrombus formation test and the flow-mediated vasodilation method. Platelet reactivity was assessed ex vivo using haemostatometry. Short-term treatment with FA markedly increased plasma folate levels and significantly suppressed laser-induced thrombus formation in apoE-/-LDLR-/- mice. Short-term treatment with FA suppressed platelet reactivity in apoE-/-LDLR-/- mice, but FA treatment did not affect endothelial function or plasma homocysteine levels. Long-term treatment with FA increased plasma folate levels dose-dependently. Thrombus formation and endothelial dysfunction were suppressed by treatment with 0.5 and 1.5mg/kg of FA, respectively, but not with 0.02mg/kg of FA, whereas platelet reactivity was not altered by treatment with any dose of FA. Long-term treatment with all doses of FA decreased the plasma homocysteine levels in apoE-/-LDLR-/- mice, although this result was not consistent with its anti-thrombotic action. In conclusion, our data showed that short- and long-term treatment with FA could suppress in vivo thrombus formation in an atherogenic setting, independent of its hypohomocysteinemic action. © 2014 Elsevier Ireland Ltd.

“個別栄養指導をする上で必要であるが自分には不足していると感じているスキル”の上位は、｢薬の知識｣や｢必要な資料を読みこなす能力｣などであった。“個別栄養指導をする上で、今後あればよいと思う学び直しプログラム”の上位は、｢カウンセリング講座｣、｢検査値に関する講座｣、「薬に関する講座」などであり、両者は必ずしも一致していなかった。本論文は臨床栄養学領域の業績である。担当部分：全般

Introduction: Prevention of arterial thrombotic diseases has high priority in developed countries. Taurine (2-aminomethylsulfonic acid), which is rich in sea foods, showed antithrombotic effect in animal models of thrombosis. The present study aimed to investigate such effect in healthy human volunteers. Methods and Results: In 101 healthy Japanese people the overall thrombotic status was accessed from non-anticoagulated blood sample by the Global Thrombosis Test (GTT). There was no significant correlation between taurine concentration in urine samples and GTT-Occlusion Times (OT; mainly reactivity of platelets). In contrast, a significant inverse correlation was demonstrated between urine taurine concentrations and GTT-Lysis Times (LT; showing spontaneous thrombolytic activity). Conclusions: Our findings suggest that taurine enhances endogenous thrombolytic activity which could be a mechanism of the earlier observed cardioprotective and antithrombotic effect. (C) 2012 Elsevier Ltd. All rights reserved.

Objective: To determine and compare thrombotic and endogenous thrombolytic status in Japanese and Western populations. Background: Incidence of coronary heart disease (CHD) and AMI in Japan remains lower than in Western countries. Primary genetic effects are unlikely, given the increased CHD in Japanese migrants. For men, cholesterol and blood pressure have been similar in Japan and the U.S. Dietary factors are implicated, but how these effect CHD is unclear. We postulated that differences in thrombotic and/or thrombolytic status may contribute. Methods: We measured thrombotic and thrombolytic status in 100 healthy Japanese (J) from Japan and 100 healthy Westerners (W) from the U.K. using the Global Thrombosis Test (GTT). The GTT employs non-anticoagulated blood to create platelet-rich thrombi under high shear (occlusion time OT: seconds), and then measures the restart of blood flow, due to spontaneous thrombolysis (lysis time LT; seconds). Results: OT was longer in (J) compared to (W) (545 vs. 364, p&lt;0.0001). LT was longer in (J) than in (W) (1753 vs. 1052, p&lt;0.0001). Distribution of LT in (J) did not conform to a normal population, with markedly impaired thrombolytic status (LT&gt;3000 s) in 18%, compared to none of the Westerners (p&lt;0.0001). Conclusions: There are marked differences in thrombotic and thrombolytic status, with (J) having less prothrombotic (longer OT) but less favourable endogenous thrombolytic profile (longer LT). This may be important in the aetiology of thrombotic events. Since platelets and thrombolysis were both inhibited in (J) and yet incidence of AMI is lower, OT would seem more important than LT as a determinant of overall thrombotic risk in this population. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

抗血栓作用を期待してハーブを用いる場合、単品のハーブを料理に用いる場合には問題にならないが、何種類かのハーブを盛り合わせる場合には、ハーブ間で作用の打消しが起こる可能性があることに注意が必要である。また、これを避けるためにもハーブの品種ごとの抗血栓作用を明らかにしておく必要がある。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：共同研究につき抽出不可能

生体電気インピーダンス分析法（Bioelectrical impedance analysis: BIA）や二重エネルギーX線吸収測定法（Dual energy X-ray absorptiometry: DEXA）は，栄養評価に重要である身体構成成分を測定算出する方法である。それぞれの原理や問題点を十分理解した上で適切に利用すれば，大変有用である。

血栓形成傾向ならびに血栓溶解活性が人種間（日本人と西欧人）で異なることを明らかにした。本論文は血液生理学領域の業績である。担当部分：共同研究につき抽出不可能

伝統的な漢方である温清飲の長期摂取は、マウスの血小板反応性抑制・血管内皮機能改善のメカニズムを介し、in vivo血栓形成傾向を有意に抑制することが示された。本論文は漢方医学（漢方による循環器疾患の予防）領域の業績である。担当部分：全般

Tetrahydrobiopterin WHO is an important cofactor for endothelial nitric oxide synthase activity. The relationship between endothelial function in vivo and aortic BH4 level is not fully understood, however. In the present study, we aimed to clarify whether reduction of aortic BH4 levels contributes to endothelial dysfunction in vivo using spontaneously hyperlipidemic mice. To estimate endothelial function in vivo and in real-time state, we developed a flow-mediated vasodilation (FMV) method in mice, which measured changes in the diameter of the femoral artery in response to increased blood flow. C57BL/6 mice and apoE/low-density lipoprotein receptor double knock-out mice were fed a low-fat diet (LFD) or a high-fat diet (HFD) for 12 weeks from 6 weeks of age. HFD feeding impaired FMV in double knockout mice, but not in C57BL/6 mice. Furthermore, HFD feeding reduced plasma NOx concentration and aortic BH4 level in double knock-out mice. Conversely, exogenous injection of BH4 (2 mg/kg) markedly increased aortic BH4 levels and restored endothelial function. In conclusion, we demonstrated that HFD feeding impaired nitric oxide-mediated endothelial function and reduced BH4 level in vivo, and that acute augmentation of aortic BH4 levels improved endothelial function. These findings indicate that BH4 is a critical determinant of nitric oxide-mediated endothelial function in hypercholesterolemia. Blood Coagul Fibrinolysis 20:699-705 (C) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.

加古郡稲美町産の地場野菜であるトマト4品種の抗血栓作用を検討した。その結果、全ての品種において血小板反応性ならびに凝固能を阻害する可能性が示唆された。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：地場作物の機能性成分分析とその応用

ポークペプチドの210mg/kg投与で有意な抗血栓作用が認められ、この抗血栓効果はアスピリン50mg/kg投与による効果と同程度であった。以上の結果から、ポークペプチドの摂取が血栓予防と治療に役立つことが示唆された。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：共同研究につき抽出不可能

The prevention of arterial thrombotic disease has a high priority in developed countries. An inappropriate diet is known to enhance the risk for acute thrombotic events and a regular diet with proven antithrombotic effects might be a beneficial way to prevent disease. The present study was undertaken as a part of a series of investigations to examine vegetables and fruits for antithrombotic activity. A shear-induced in vitro platelet reactivity/thrombolysis method [Global Thrombosis Test, formerly Gorog Thrombosis Test] was used to screen 15 different varieties of carrot for antiplatelet and thrombolytic activities. Effective varieties were further investigated using an in vivo, laser-induced thrombosis model in mice. The different carrot varieties demonstrated a variable effect on thrombosis in vitro and in vivo. In particular, a variety designated SAKATA-0421 exerted an antithrombotic effect in vivo independent from heat treatment of the filtrate at 100 degrees C for 10 min. There was no significant correlation between antithrombotic activity and the levels of polyphenolics and any other biochemical parameter, including antioxidant activity, alpha-carotene and beta-carotene, alpha-tocopherol and ascorbic acid. Different varieties of carrot demonstrated a range of antithrombotic and prothrombotic activities. After oral intake, the particular heat resistant variety (SAKATA-0421) showed antithrombotic effect in vivo possibly due to antiplatelet reactivity and/or spontaneous thrombolytic activity. The present study added a new variety to the list of antithrombotic fruits and vegetables. Blood Coagul Fibrinolysis 19:785-792 (C) 2008 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

Epidemiological evidence exists for a causative role of inappropriate diets in the development and clinical outcome of thrombotic diseases. Our previous studies have shown that some fruits and vegetables have antithrombotic activity. As rice is a principal ingredient of everyday food in Japan, the aim of the present study was to determine rice varieties with antithrombotic effect. Western-style high-fat diet containing flour prepared from whole-grain rice of different varieties was fed to atherosclerosis-prone (apolipoprotein E- and low density lipoprotein receptor-deficient) mice for 3 months. A laser-induced carotid-artery thrombosis model was used. None of the five tested rice varieties showed an antithrombotic effect. The effect of HYJA-Ri1, HYJA-Ri2, HYJA-Ri3 and HYJA-Ri5 rice on experimental thrombus formation did not differ significantly from the controls. However, HYJA-Ri4 rice significantly enhanced the thrombotic reaction (p = 0.0078), thus having a prothrombotic effect. Our findings suggest that HYJA-Ri4 rice should not be included in an antithrombotic diet. Copyright © 2009 S. Karger AG, Basel.

食餌感受性の高いHe-Neレーザー惹起マウス頚動脈血栓形成法を確立した。本法は、種々の食品成分の抗血栓作用測定を可能にした。ジアシルグリセロールは血栓形成傾向の増大を抑制したが、この効果は血小板機能や凝固能ではなく、血管内皮機能への影響を介するものであることが明らかされた。さらに、今回開発されたin vivo法とズリ惹起血小板反応性/血栓溶解能in vitro測定法の組み合わせで、抗血栓作用をもつ野菜・果物の品種探索が可能なことが示唆された。本研究では細胞レベルでの生物学的なアプローチが採用されたが、このアプローチは血栓症の一次予防に必要な抗血栓作用を持つ野菜や果物のスクリーニングだけでなく、抗血栓作用の分子メカニズムの解明においても有益と考えられた。Ａ４判 全60頁

Prevention of arterial thrombotic disease has high priority in developed countries. As inappropriate diet predispose to acute thrombotic events, regular intake of an antithrombotic diet may be a convenient and effective way of prevention. The present study was performed to examine antithrombotic effect of mulberry varieties. A shear-induced in vitro platelet reactivity/thrombolysis test (Gorog Thrombosis Test) was used to screen for antiplatelet and thrombolytic activities. In case of effectiveness, it was followed by an in vivo test of laser-induced thrombosis in mice. Antioxidant capacity was assessed by superoxide anion and radical scavenging activities. Total polyphenolics, anthocyanin and citrate contents were also measured. The tested varieties showed different effect in vitro on platelet reactivity and endogenous thrombolytic activity. Varieties inhibiting platelet reactivity were antithrombotic in vivo regardless inhibition or enhancement of thrombolysis. Those mulberry varieties, which enhanced platelet reactivity in vitro, were prothrombotic only if inhibitory activity on endogenous thrombolysis coexisted with the platelet effect. Antioxidant activities and polyphenolics content did not affect platelets and the overall thrombotic status. However, antioxidant activities and polyphenolics content significantly correlated with the endogenous thrombolytic activity. These data showed that mulberry varieties can be grouped into subclasses with either anti- or prothrombotic activities. Antioxidant activities and polyphenolic contents do not affect platelets but may enhance endogenous thrombolysis, thus causing an overall antithrombotic effect.

Introduction: We have shown earlier that diacylglycerol (DAG) but not triacylglycerol (TAG) inhibited thrombus formation. The aim of the present study was to investigate the mechanism of this antithrombotic effect of DAG. Materials and Methods: Four different diets, the (1) Western-style high-fat diet (HFD) containing 20% lipid and 0.05% cholesterol (w/w), (2) TAG-rich and (3) DAG- rich HFDs containing 20% lipid and 0.05% cholesterol, but all lipid replaced by TAG or DAG oil with very similar fatty acid composition and the (4) Japanese-style low-fat diet (LFD) containing 7% oil but no cholesterol were given to apolipoprotein E and low-density lipoprotein (LDL) receptor double-deficient mice. Atherogenicity was assessed by morphology, mapping the whole aorta and measuring the total area of lipid-stained lesions. Endothelial function was measured by the flow-mediated vasodilation test. Platelet reactivity was assessed from native blood sample by a shear-induced platelet function test (hemostatometry). Serum lipoprotein profile was measured by HPLC. Results: Both the Western-style and the TAG-rich HFDs have accelerated atherosclerosis. In contrast, DAG-rich HFD inhibited the atherosclerotic process to an extent comparable with the Japanese-style LFD. There was no significant difference in platelet and coagulant activity between the studied diet groups. DAG-rich but not the TAG-rich HFD significantly suppressed serum LDL cholesterol level. Conclusions: The present findings suggest that the mechanism of antithrombotic and anti-atherogenic effect of DAG may involve the protection of the vascular endothelium from injury and lowered serum LDL cholesterol. Copyright (c) 2006 S. Karger AG, Basel.

Introduction: Earlier we have demonstrated a prothrombotic state in spontaneously atherogenic rodents kept on Western-style high fat diet. The aim of the present study was to investigate the cellular mechanism of such prothrombotic state. Materials and methods: Two kinds of diets, Western-style high fat diet containing 20% fat (w/w) and 0.05% cholesterol (w/w) and low fat diet containing 7% fat without cholesterol based on AIN93G, were added to diet-sensitive apolipoprotein E and low-density lipoprotein receptor double deficient mate mice for 12 or 18 weeks from 6 weeks of age. Atherosclerosis was assessed by morphometry of the aortic watt or lipid-stained lesions. Endothelial function was measured by flow-mediated vasodilation (FMV) of the femoral artery. Platelet reactivity was measured ex vivo by a shear-induced platelet aggregation test. Results and conclusions: 12 weeks feeding of mice with high fat diet significantly impaired FMV, as compared with mice fed with low fat diet (P &lt; 0.05). In contrast, there was no significant difference in the lipid-stained areas and in the reactivity of platelets between the two groups. 18 weeks feeding with high fat diet significantly impaired FMV (P &lt; 0.05) and enhanced both lipid-stained areas (P &lt; 0.05) and platelet reactivity (P &lt; 0.01). These findings show that in high fat diet-induced prothrombotic state, endothelial dysfunction precedes both the morphologically detectable lesions and the enhancement of platelet reactivity. (c) 2005 Elsevier Ltd. All rights reserved.

先天的動脈硬化発症ApoE-/- LDLR-/- ダブルノックアウトマウスの頸動脈とHe-Neレーザー惹起血栓形成法を組み合わせた、食餌感受性の高い血栓のマウスモデルを用い、植物性ならびに動物性タンパク質の有用性を検討した。その結果、分離大豆タンパク質は血栓形成傾向を抑制した。またこの抑制は、動脈硬化形成過程を抑制することによることが示唆された。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：共同研究につき抽出不可能

Introduction: Prevention of arterial thrombotic diseases has high priority in developed countries. An appropriate diet is thought to be the best way to prevent or reduce the risk of mortality from such diseases. The aim of the present study was to evaluate the effect of diacylglycerol (DAG)-rich diets on arterial thrombosis. Material and methods: Diet-sensitive congenital apolipoprotein E (ApoE) and LDL receptor (LDLR) double deficient mice were used. Thrombosis was assessed by the rate and extent of thrombus formation in the carotid artery of mice after laser irradiation. Plasma total cholesterol and triglyceride levels were measured by enzymatic assays. Four kinds of diets were used: high fat (Western-style) diet contained 20% fat (w/w) and 0.05% cholesterol (w/w); the TAG-rich and the DAG-rich high fat diet contained 20% TAG or DAG oil (w/w) with very similar fatty acid composition and 0.05% cholesterol; Low fat (Japanese-style) diet contained 7% fat, without cholesterol. These diets were on the basis of AIN93G and were given to mice for 8 weeks from 6 weeks of age. Results: Western-style high fat and TAG-rich high fat diets significantly increased thrombogenicity compared with low fat diet. DAG-rich high fat diet showed the lowest value, and the extent of thrombogenicity was equivalent to the low fat diet group. Fasting plasma total cholesterol level of DAG-rich high fat and low fat diet groups were significantly lower than that of TAG-rich high fat and high fat diet groups. Fasting plasma triglyceride Levels in DAG-rich high fat diet group were significantly lower than in the TAG-rich high fat diet group. Conclusions: Dietary DAG but not TAG oil extenuates arterial thrombus formation. The mechanism of this effect is unclear and further investigated. (C) 2005 Elsevier Ltd. All rights reserved.

Objective: Obesity is a common risk factor in insulin resistance and cardiovascular diseases. Although hypoadiponectinemia is associated with obesity-related metabolic and vascular diseases, the role of adiponectin in thrombosis remains elusive. Methods and Results: We investigated platelet thrombus formation in adiponectin knockout (APN-KO) male mice (8 to 12 weeks old) fed on a normal diet. There was no significant difference in platelet counts or coagulation parameters between wild-type (WT) and APN-KO mice. However, APN-KO mice showed an accelerated thrombus formation on carotid arterial injury with a He-Ne laser (total thrombus volume: 13.36 +/- 4.25 x 10(7) arbitrary units for APN-KO and 6.74 +/- 2.87 x 10(7) arbitrary units for WT; n = 10; P &lt; 0.01). Adenovirus-mediated supplementation of adiponectin attenuated the enhanced thrombus formation. In vitro thrombus formation on a type I collagen at a shear rate of 250 s(-1), as well as platelet aggregation induced by low concentrations of agonists, was enhanced in APN-KO mice, and recombinant adiponectin inhibited the enhanced platelet aggregation. In WT mice, adenovirus-mediated overexpression of adiponectin additionally attenuated thrombus formation. Conclusion: Adiponectin deficiency leads to enhanced thrombus formation and platelet aggregation. The present study reveals a new role of adiponectin as an endogenous antithrombotic factor.

本研究の目的は、栄養系学部教育において、協調学習の推進を目的として、電子掲示板上で解剖生理学の科目に関連した学習ディスカッション（Web forum）を行い、その有用性を明らかにすることである。学生を4人ずつに編成し、1グループにひとつ、解剖学に関するテーマについてのレポートを課した。提出されたレポートを電子掲示板にリンクさせ、その内容について学生に自由な発言を促した。本研究で構築したWeb Forum法は学生の主体性と学習能力を高め、学習内容に深みを持たせる点で、コ・メディカル教育に有用であることが示唆された。本論文は情報処理学領域の業績である。担当部分：授業の補助・アンケートの実施等

4名の被験者に人為的に椋鳥住血吸虫セルカリア（Gigantobilharzia sturniae；以下Gsセルカリアと略）を前腕に接種して、発疹の肉眼的経過・血中抗体価・皮内反応を観察すると共に、Gsセルカリアがヒト皮膚にどのように侵入し、どのように変化していくか病理組織の変化を観察した。経日的に発疹の生検を行って、小田琢三博士による実験的皮膚炎病理所見との比較検討を行った。本論文は微生物学領域の業績である。担当部分：顕微鏡写真の撮影、論文資料作成等

嗅覚刺激が人体にどのような影響を及ぼすかについて、とくに脳波、自律神経、ならびに体表面の温熱変化を中心し検討を加えた。その結果、嗅覚刺激を行うことによって、脳波にかなりの変化をもたらすことが確認された。すなわち、通常緊張時にみられるβ波の出現率が少なく、むしろθ波やα波が優勢にみられたが、これは心地よい嗅覚刺激によってえられた事実が原因であることが立証された。本論文は生理学領域の業績である。担当部分：共同研究につき抽出不可能

Because of the high mortality, prevention of arterial thrombotic disease has top priority in developed countries. As inappropriate diet is known to predispose to acute thrombotic events, regular intake of an anti-thrombotic diet may offer a convenient and effective method of prevention. As part of a systematic investigation into the anti-thrombotic effect of fruits and vegetables, strawberry varieties were tested in this study. An in vitro platelet function test (haemostatometry) was used for screening strawberry filtrates. Those that showed significant antiplatelet effect were further assessed with a laser-induced thrombosis test in mice. Measurement of flow-mediated vasodilation in the femoral artery of mice reflected the effect on the vascular endothelium. Correlation between the effects on platelet reactivity in vitro and the antioxidant activity (hypoxanthine/ xanthine oxidase test) or phenolic compound content was assessed. Strawberry varieties KYSt-4 (Nohime), KYSt-11 (Kurume IH-1) and KYSt-17 (Kurume 58) showed significant antiplatelet activity both in vitro and, after oral administration, in vivo. Both KYSt-11 and KYSt-17, but not KYSt-4, significantly reduced flow-mediated vasodilation that is, caused endothelial dysfunction. Antiplatelet activities were heat stable. Significant correlation was found between antiplatelet and antioxidant activities (P = 0.049, R2 = 0.23) or total phenolic compounds (P = 0.0096, R 2 = 0.36). Of the tested strawberry varieties, KYSt-4, KYSt-11 and KYSt-17 showed significant anti-thrombotic effect. The dual mechanism of the effect may involve a direct inhibition of both platelet function and antioxidant activities. © 2005 Lippincott Williams &amp Wilkins.

n-3系脂肪酸の作用が注目されている。そこで、先天的動脈硬化発症ApoE-/- LDLR-/- ダブルノックアウトマウスの頸動脈とHe-Neレーザー惹起血栓形成法を組み合わせた、食餌感受性の高い血栓のマウスモデルを用い、n-3系脂肪酸の有用性を検討した。その結果、n-3系脂肪酸増量の割合に依存して抗血栓効果が認められた。この成績はn-3系脂肪酸がn-6系脂肪酸によるアラキドン酸由来の種々のエイコサノイド産生抑制をもたらし、血栓形成傾向を抑制した可能性を示唆した。本論文は臨床栄養学（食事による循環器疾患の予防）領域の業績である。担当部分：共同研究につき抽出不可能