Maki Ashida

Introduction: The next generation surface modification on medical and dental implants is coatings of tissue or stem cells on them.1 To promote cellular attachment and formation of tissues on materials, the control of patterned surface topography is necessary, as the surface topography of a material governs its biocompatibility. For example, in the case of titanium (Ti), nanometer scale topographical features influence cell spreading and micrometer scale topography promotes stem cell differentiation in vitro.2-4 To clarify the role of patterned nano topographies on the biocompatibility and osteoconductivity of metal implant, we investigated the adhesion and calcification of mouse preosteoblast (MC3T3-E1) to Ti surfaces with three surface patterned nano-ripples topography fabricated by single-shot femtosecond laser irradiation.

Micro-arc oxidation (MAO) was performed on a titanium (Ti) to achieve both hard-tissue compatibility and antibacterial property. The surface oxide layer formed on Ti by MAO treatment in a mixture of calcium glycerophosphate, calcium acetate, and silver nitrate was evaluated by surface analyses, cell and microbial testing. Small amount of Ag was incorporated into the porous oxide layer by MAO treatment. Ag-ion release was confirmed during immersion in a physiological saline. The released Ag ions completely inhibit the proliferation of E. coli while the proliferation and mineralization activity of MC3T3-E1 cells was not affected when MAO treatment condition was optimized.