戸谷 希一郎

In molecular biology research, a vitamin E (VE) vehicle (VE dissolved in organic solvent) is often added to water media without a stabilizer. However, the detailed behavior of VE colloids in water media is unclear. In this study, we reveal that VE nanoemulsion readily forms in water-based media through the existing protocol. The colloid size was changed from 39 nm to the submicron scale by adjusting the initial concentration of the VE solution and adding a buffer. The radical scavenging effect of the dispersed nanosized VEs is comparable to that of the water-soluble antioxidant Trolox, providing excellent antioxidant performance in colloid form. The cytoprotection effect of the VE colloids under a lipid oxidation condition largely depends on the size of the nanodispersion. Smaller dispersed particles are more efficient radical scavengers than larger particles for a constant VE amount owing to sophisticated uptake behavior of cell. This unveiled fundamental knowledge pave the way for a preparative protocol of stabilizer-free VE vehicles, which are expected to become widely used in molecular biology research.

Glycoprotein folding plays a critical role in sorting glycoprotein secretion and degradation in the endoplasmic reticulum (ER). Furthermore, relationships between glycoprotein folding and several diseases, such as type 2 diabetes and various neurodegenerative disorders, are indicated. Patients’ cells with type 2 diabetes, and various neurodegenerative disorders induce ER stress, against which the cells utilize the unfolded protein response for protection. However, in some cases, chronic and/or massive ER stress causes critical damage to cells, leading to the onset of ER stress-related diseases, which are categorized into misfolding diseases. Accumulation of misfolded proteins may be a cause of ER stress, in this respect, perturbation of oligomannose-type glycan processing in the ER may occur. A great number of studies indicate the relationships between ER stress and misfolding diseases, while little evidence has been reported on the connection between oligomannose-type glycan processing and misfolding diseases. In this review, we summarize alteration of oligomannose-type glycan processing in several ER stress-related diseases, especially misfolding diseases and show the possibility of these alteration of oligomannose-type glycan processing as indicators of diseases.