高野 温子

HERBARIUM PLANT SPECIMENS AND THEIR HERBARIUM HOLDINGS Natural history collections around the globe represent a dormant source of taxonomic and biogeographic information, as it is estimated that one to three billion specimens are included in such collections (Soberon 1999; Ariño 2010). In the case of botanical collections, the worldwide distribution of specimens across herbaria is often uneven and biased by colonialism, with most specimens housed in the global North, and in many cases resulting in a reverse latitudinal relationship between plant diversity present in nature and the location of the herbaria housing it (Park et al. 2023). As an example, Yong (2013) reviewed holdings of common and type specimens and concluded that the type specimens’ accumulation in China is less than the world average. The number of type specimens is an important reflection of botanical accumulation in a country, and the number of specimens and their origins are therefore very important in a globalized framework. Accordingly, efforts to catalogue and digitize collections, and to make them globally accessible, are important tasks for herbaria. HERBARIA IN JAPAN AND THE UNIVERSITY OF TOKYO (TI) HERBARIUM In Japan, 74 herbaria are registered in Index Herbariorum (Thiers 2024), accounting for more than 13 million plant specimens. Herbaria in Japan date from the 19th century, with the first herbarium founded in Japan being the University of Tokyo’s (TI) herbarium (Herbarium of the University of Tokyo 2021) established in 1877. The herbarium holds a collection of more than 1,800,000 specimens of vascular plants, corresponding to ca. 14% of the total botanical collection of Japan. Although there is no complete survey of the number of specimens in the collection, it is estimated that the TI herbarium holds 20,000 type specimens, corresponding to 1% of the total number in the collection. Within the type specimens, only a fraction of the collection is digitized and available online, including 3,793 type specimens of angiosperms (and 1,743+ type specimens from other groups newly photographed in the last year). Regarding the ferns and lycophytes, the TI herbarium holds 35 families, including more than 300,000 specimens, comprising 1068+ type specimens (with 321 confirmed holotypes, 127 isotypes, and 510 syntypes), which are now being databased. The TI herbarium fern and lycophyte type collection includes plants from Japan, and important collections from Korea, Manchuria, Taiwan, and many Southeast Asian countries. Collections from outside Japan were mainly gathered by Japanese botanists that were assigned to expeditions in those countries, as Japan’s national power increased rapidly after the Meiji restoration (1869–1889). For example, Takiya Kawakami, originally from the Hokkaido University, actively worked on collecting plants from Taiwan; Hiroshi Hara worked on the Himalayan flora; Takenoshin Nakai worked on the Korean flora and collected medicinal plants from Indonesia and Ceylon (Sri Lanka); and Bunzo Hayata made a huge contribution to Taiwan’s flora. The specimens collected hold not only information on the taxonomic diversity, but also reflect historic events between Japan and these adjacent countries, thus proper curation of these materials is of great importance. The TI herbarium also holds unique collections, including many fern specimens collected from Seram Island (Indonesia), South America’s Andes collections by Fumio Maekawa, and Brazil’s Amazon collections by Masayuki Takeuchi. THE HIDDEN POTENTIAL OF THE TI HERBARIUM AND FUTURE PROSPECTS The TI herbarium collection is an important resource of natural history information, but its potential is still hindered by its incomplete databasing. In the Global Biodiversity Information Facility (GBIF) database, the Tracheophyta data from Japan currently consists of 3,503,623 herbarium specimens (GBIF.org 2024). Most of the data is from the TNS herbarium (500,060 specimens, National Museum of Nature and Science 2024), Kanagawa Prefectural Museum of Natural History (318,574 specimens, Kanagawa Prefectural Museum of Natural History 2024) and Nagano Environmental Conservation Research Institute (170,889 specimens, Nagano Prefecture 2023). The University of Tokyo's TI herbarium data only contains 40,382 specimens, not including any ferns or lycophytes, restricting access to the collection for researchers abroad. The TI herbarium mission now is to properly catalogue and make that information available, especially to those based in regions from where the plants were originally collected. Since 1981, many scientists have contributed to the cataloguing of the type specimens in the TI herbarium, with some of this data made available online. However, it is estimated that this database covers less than 10% of the collection, and does yet not include the ferns or lycophytes. We see the development of an open-access database for the TI herbarium as of utmost importance, as the herbarium represents an important historical and biodiversity account for East Asia.

The new collection building named Collectionarium*1 (CN) of the Museum of Nature and Human Activities, Hyogo, (HYO), Hitohaku*2 Japan, which opened in October 2022, contains ca. 600,000 vascular plant specimens, including 10% that were not mounted. Here, we describe the process of specimen moving to the CN, including integration with ca. 250,000 herbarium specimens of Shoei Junior College (known as SHO). At the same time, the collection sequence has been updated from the new Engler system (Melchior 1964) to the Angiosperm Phylogeny Group (APG) III system. The storage rooms of natural history museums are full everywhere across the world, and extensions are necessary. When collections spaces are enlarged, it creates opportunities to relocate specimens. We hope that our experiences will be useful to other institutions. The Status of the HYO Collection in 2020 When the construction of the CN started in early 2020, we had 600,000 plant specimens: ca. 350,000 on 220 shelves and the other 250,000 in ca. 1,500 cardboard boxes. The 250,000 specimens, donated by the SHO in 2012, were kept in boxes as existing shelves were too short to house them. Based on an estimated capacity of 1,000–2,000 specimens per shelf, the new storage consisting of 525 shelves would not have the capacity to store all the specimens. Therefore, it was necessary to estimate the total volume of specimens more accurately. The classification system also needed updating. Change From Engler to APG At the time of its opening in 1992, HYO specimens were arranged according to the new Engler system. However, the APG system began to be adopted more recently. The new Engler and APG systems are quite distinct, treating dicots in a different way, and some families have disappeared and new ones arisen. The plant picture books published recently in Japan all adopt the APG system. The younger generation studying plants never learned the new Engler system, making it impossible for them to find specimens arranged in this way. Therefore, we decided to change the arrangement of the specimens to the APG III system whilst relocating the herbarium. For pteridophytes, we decided to adopt the Pteridophyte Phylogeny Group (PPG) I system (PPG I 2016). In order to plan the placement of specimens, it was necessary to know the number of specimens at the genus level and to investigate how much space each family would need. We measured the thickness of all genus covers containing specimens, regardless of whether they were in shelves or boxes. Based on these results, we planned the layout. Next we packed the specimens into cardboard boxes following the APG system, and two surfaces of each box was labeled with the room number (=1 or 2) in the CN with locations in the room. All the boxes were moved to the Holonpia Hall (the Hall) from December 2021 to January 2022. Then, 220 empty specimen shelves were transported to the CN in February 2022. After the shelves were moved, ca. 4,000 boxes stored in the Hall were carried back into old repository in March 2022 to wait for moving. Moving to the new storage Gases, e.g., ammonia, are released from concrete buildings for a while after construction. Since these gases are harmful to specimens, it is necessary to wait until the gas concentration decreases to a safe level, before storing the specimens there. Beginning in April 2022, the ammonia concentration was measured once a month in the two new storage rooms at the CN. Rooms were ventilated nearly every day. In November 2022, the concentration of ammonia in both rooms fell to 30 ppb or less and it was finally safe to move the specimens in. Moving was carried out during the regular maintenance closure in January 2023. First, we carried the boxes to the exhibition area on the first floor of the CN because we needed enough space for unpacking and sorting. We opened boxes one by one for each family to combine the HYO and SHO specimens, sorted them in alphabetical order, re-packed them into boxes, brought the boxes up to the storage rooms on the second floor, and placed them onto the shelves. Two teams of people, three or four for each storage room, worked with this system for six days until all the specimens were placed, properly arranged, and on the shelves by late January. Specimens are now arranged according to the APG system, and ready to be examined. Please contact the authors for herbarium study requests.