研究者業績

緒方 英明

オガタ ヒデアキ  (Hideaki Ogata)

基本情報

所属
兵庫県立大学 大学院理学研究科 教授
学位
博士(理学)(京都大学)

研究者番号
30795935
ORCID ID
 https://orcid.org/0000-0002-2894-2417
J-GLOBAL ID
201701006048438970
Researcher ID
J-4975-2013
researchmap会員ID
B000274859

受賞

 1

論文

 77
  • 緒方英明
    日本結晶学会誌 65(4) 220-221 2023年12月15日  査読有り招待有り筆頭著者責任著者
  • Takahiro Sakai, Tsuyoshi Mashima, Naoya Kobayashi, Hideaki Ogata, Lian Duan, Ryo Fujiki, Kowit Hengphasatporn, Taizo Uda, Yasuteru Shigeta, Emi Hifumi, Shun Hirota
    Nature communications 14(1) 7807-7807 2023年12月8日  査読有り
    Overexpression of antibody light chains in small plasma cell clones can lead to misfolding and aggregation. On the other hand, the formation of amyloid fibrils from antibody light chains is related to amyloidosis. Although aggregation of antibody light chain is an important issue, atomic-level structural examinations of antibody light chain aggregates are sparse. In this study, we present an antibody light chain that maintains an equilibrium between its monomeric and tetrameric states. According to data from X-ray crystallography, thermodynamic and kinetic measurements, as well as theoretical studies, this antibody light chain engages in 3D domain swapping within its variable region. Here, a pair of domain-swapped dimers creates a tetramer through hydrophobic interactions, facilitating the revelation of the domain-swapped structure. The negative cotton effect linked to the β-sheet structure, observed around 215 nm in the circular dichroism (CD) spectrum of the tetrameric variable region, is more pronounced than that of the monomer. This suggests that the monomer contains less β-sheet structures and exhibits greater flexibility than the tetramer in solution. These findings not only clarify the domain-swapped structure of the antibody light chain but also contribute to controlling antibody quality and advancing the development of future molecular recognition agents and drugs.
  • Takeshi Hiromoto, Koji Nishikawa, Seiya Inoue, Hideaki Ogata, Yuta Hori, Katsuhiro Kusaka, Yu Hirano, Kazuo Kurihara, Yasuteru Shigeta, Taro Tamada, Yoshiki Higuchi
    Chemical Science 14(35) 9306-9315 2023年9月21日  査読有り
  • Ami Kobayashi, Midori Taketa, Keisei Sowa, Kenji Kano, Yoshiki Higuchi, Hideaki Ogata
    IUCrJ 10(5) 544-554 2023年9月1日  査読有り
  • Nipa Chongdar, Patricia Rodríguez-Maciá, Edward J. Reijerse, Wolfgang Lubitz, Hideaki Ogata, James A. Birrell
    Chemical Science 14(13) 3682-3692 2023年4月7日  査読有り責任著者

MISC

 12

書籍等出版物

 6

主要な講演・口頭発表等

 20

共同研究・競争的資金等の研究課題

 2