CVClient

柳澤 幸子

Sachiko YANAGISAWA

基本情報

所属
兵庫県立大学 大学院 理学研究科 准教授
学位
Doctor of Philosophy(2006年7月 Faculty of Science, Agriculture and Engineering University of Newcastle upon Tyne)

J-GLOBAL ID
201801003242745601
researchmap会員ID
B000329623

論文

 64
  • Sachiko Yanagisawa, Takuto Kamei, Atsuhiro Shimada, Stephanie Gladyck, Siddhesh Aras, Maik Hüttemann, Lawrence I. Grossman, Minoru Kubo
    Journal of Inorganic Biochemistry 260 112673-112673 2024年11月  
  • Kaho Ueda, Wataru Sato, Sachiko Yanagisawa, Minoru Kubo, Masahiko Hada, Hiroshi Fujii
    Journal of Inorganic Biochemistry 255 112544-112544 2024年6月  査読有り
  • Yoshiaki Kokubo, Kazuki Tsuzuki, Hikari Sugiura, Shunsuke Yomura, Yuko Wasada-Tsutsui, Tomohiro Ozawa, Sachiko Yanagisawa, Minoru Kubo, Tomoyuki Takeyama, Takahide Yamaguchi, Yuichi Shimazaki, Shinichi Kugimiya, Hideki Masuda, Yuji Kajita
    Inorganic Chemistry 62(14) 5320-5333 2023年3月27日  
  • Mohan Zhang, Hulin Tai, Sachiko Yanagisawa, Masaru Yamanaka, Takashi Ogura, Shun Hirota
    The Journal of Physical Chemistry B 127(11) 2441-2449 2023年3月15日  査読有り
  • Yuya Nishida, Sachiko Yanagisawa, Rikuri Morita, Hideki Shigematsu, Kyoko Shinzawa-Itoh, Hitomi Yuki, Satoshi Ogasawara, Ken Shimuta, Takashi Iwamoto, Chisa Nakabayashi, Waka Matsumura, Hisakazu Kato, Chai Gopalasingam, Takemasa Nagao, Tasneem Qaqorh, Yusuke Takahashi, Satoru Yamazaki, Katsumasa Kamiya, Ryuhei Harada, Nobuhiro Mizuno, Hideyuki Takahashi, Yukihiro Akeda, Makoto Ohnishi, Yoshikazu Ishii, Takashi Kumasaka, Takeshi Murata, Kazumasa Muramoto, Takehiko Tosha, Yoshitsugu Shiro, Teruki Honma, Yasuteru Shigeta, Minoru Kubo, Seiji Takashima, Yasunori Shintani
    Nature Communications 13 7591 2022年12月8日  査読有り
    Abstract Antimicrobial resistance (AMR) is a global health problem. Despite the enormous efforts made in the last decade, threats from some species, including drug-resistant Neisseria gonorrhoeae, continue to rise and would become untreatable. The development of antibiotics with a different mechanism of action is seriously required. Here, we identified an allosteric inhibitory site buried inside eukaryotic mitochondrial heme-copper oxidases (HCOs), the essential respiratory enzymes for life. The steric conformation around the binding pocket of HCOs is highly conserved among bacteria and eukaryotes, yet the latter has an extra helix. This structural difference in the conserved allostery enabled us to rationally identify bacterial HCO-specific inhibitors: an antibiotic compound against ceftriaxone-resistant Neisseria gonorrhoeae. Molecular dynamics combined with resonance Raman spectroscopy and stopped-flow spectroscopy revealed an allosteric obstruction in the substrate accessing channel as a mechanism of inhibition. Our approach opens fresh avenues in modulating protein functions and broadens our options to overcome AMR.

MISC

 4

講演・口頭発表等

 10

共同研究・競争的資金等の研究課題

 4