University of Hyogo Academic Staff search

Yuri Nishino

  (西野 有里)

Profile Information

Affiliation
Graduate School of Life Science, University of Hyogo
Degree
薬学修士(大阪大学大学院薬学研究科)
博士(理学)(兵庫県立大学大学院生命理学研究科)

J-GLOBAL ID
200901096220712031
researchmap Member ID
5000072128

Papers

 49
  • Atsushi Ohma, Kazuki Arihara, Tetsuya Mashio, Yoshiko Ito, Yuri Nishino, Atsuo Miyazawa
    International Journal of Electrochemical Science, 19(5) 100539-100539, May, 2024  Peer-reviewed
  • Masamitsu Wada, Takeshi Higa, Kaoru Katoh, Nobuko Moritoki, Tomonori Nakai, Yuri Nishino, Atsuo Miyazawa, Shinsuke Shibata, Yoshinobu Mineyuki
    Journal of plant research, Apr 10, 2024  Peer-reviewed
    Chloroplast-actin (cp-actin) filaments are crucial for light-induced chloroplast movement, and appear in the front region of moving chloroplasts when visualized using GFP-mouse Talin. They are short and thick, exist between a chloroplast and the plasma membrane, and move actively and rapidly compared to cytoplasmic long actin filaments that run through a cell. The average period during which a cp-actin filament was observed at the same position was less than 0.5 s. The average lengths of the cp-actin filaments calculated from those at the front region of the moving chloroplast and those around the chloroplast periphery after stopping the movement were almost the same, approximately 0.8 µm. Each cp-actin filament is shown as a dotted line consisting of 4-5 dots. The vector sum of cp-actin filaments in a moving chloroplast is parallel to the moving direction of the chloroplast, suggesting that the direction of chloroplast movement is regulated by the vector sum of cp-actin filaments. However, once the chloroplasts stopped moving, the vector sum of the cp-actin filaments around the chloroplast periphery was close to zero, indicating that the direction of movement was undecided. To determine the precise structure of cp-actin filaments under electron microscopy, Arabidopsis leaves and fern Adiantum capillus-veneris gametophytes were frozen using a high-pressure freezer, and observed under electron microscopy. However, no bundled microfilaments were found, suggesting that the cp-actin filaments were unstable even under high-pressure freezing.
  • Koichiro Oishi, Mayu Nagamori, Yasuhiro Kashino, Hiroshi Sekiguchi, Yuji C. Sasaki, Atsuo Miyazawa, Yuri Nishino
    International Journal of Molecular Sciences, 24(15), Jul 28, 2023  Peer-reviewedLast authorCorresponding author
    Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that play an important role in signal transduction at the neuromuscular junction (NMJ). Movement of the nAChR extracellular domain following agonist binding induces conformational changes in the extracellular domain, which in turn affects the transmembrane domain and opens the ion channel. It is known that the surrounding environment, such as the presence of specific lipids and proteins, affects nAChR function. Diffracted X-ray tracking (DXT) facilitates measurement of the intermolecular motions of receptors on the cell membranes of living cells, including all the components involved in receptor function. In this study, the intramolecular motion of the extracellular domain of native nAChR proteins in living myotube cells was analyzed using DXT for the first time. We revealed that the motion of the extracellular domain in the presence of an agonist (e.g., carbamylcholine, CCh) was restricted by an antagonist (i.e., alpha-bungarotoxin, BGT).
  • Yuri Nishino, Yoshiko Ito, Atsuo Miyazawa
    57(3) 139-144, Dec, 2022  InvitedLead author
  • Junichi Shimanuki, Hideto Imai, Yoshiko Ito, Yuri Nishino, Atsuo Miyazawa
    Microscopy (Oxford, England), Nov 17, 2022  Peer-reviewed
    It is important to understand and control the fine structure of the fuel cell catalyst layer in order to improve the battery characteristics of the fuel cell. A major challenge in observing the microstructure of the catalyst layer by electron microscopy is the visualization of ionomers, which have low contrast and are susceptible to damage by electron beam irradiation. Previous papers have reported transmission electron microscopy (TEM) observations of ionomers neutralized with cesium (Cs) ions. However, this approach involves chemical reactions and an indirect visualization of ionomers. In contrast, we have previously revealed the microstructure of ionomers in frozen catalyst inks by cryogenic scanning electron microscopy (cryo-SEM) and cryo-TEM. In general, ionomers are basically used under high temperature and humid conditions while the fuel cell is operating. Therefore, in this study, ultrathin sections prepared from the fuel cell catalyst layer (membrane electrode assemblies) were incubated in the chamber at high temperature and humid conditions and then rapidly frozen for observation by cryo-TEM. As a result, we succeeded in observing the pore structure of the catalyst layer in the swollen state of the ionomer. The swollen ionomer surrounded and enclosed the Pt/C aggregates and bridged over the pores in the catalyst layer.

Misc.

 31

Books and Other Publications

 1

Presentations

 15

Research Projects

 7

Industrial Property Rights

 1

Academic Activities

 2