基本情報
- 所属
- 日本獣医生命科学大学 獣医学部 獣医保健看護学科 応用部門 教授
- 学位
- the degree of doctor of veterinary science(Nippon Veterinary and Life Science University)the degree of Master of veterinary science(Gifu University)
- J-GLOBAL ID
- 200901011389859990
- researchmap会員ID
- 1000093587
経歴
8-
2011年4月 - 現在
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1996年4月 - 2011年3月
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1988年 - 1996年
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1988年 - 1996年
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1980年 - 1988年
学歴
4-
- 1980年
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- 1980年
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- 1978年
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- 1978年
論文
16-
Journal of Comparative Pathology 201 28-32 2023年2月
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Veterinary Pathology 030098582110186-030098582110186 2021年5月31日Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.
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Severe Jackknife-like Kyphosis Malformation in the Fetus of a Free-ranging Sika Deer (Cervus nippon)Japanese Journal of Zoo and Wildlife Medicine 25(4) 141-145 2020年12月24日
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Veterinary Pathology 56(3) 389-398 2019年3月 査読有り
MISC
43-
日本獣医生命科学大学研究報告 (65) 34-38 2016年12月1日A male, mixed breed, over 8 years old dog had a mass palpated in the right cryptochidism located at the inguinal subcutaneous region. At necropsy another large mass was observed in the retroperitoneum. The masses in the right testis and in the retroperitoneum were approximately 7.5 cm and 10.0 cm in diameter, respectively. Histopathological examination revealed that both masses were composed of polygonal cells with moderate to high grade atypia. The tumor cells formed alveolar or cord structures separated by fibrous stroma, and showed a tendency to orient perpendicularly to the basement membrane. Immunohistochemically, the tumor cells were positive for vimentin, inhibin-α and WT1. The testis was diagnosed with malignant Sertoli cell tumor, and the mass in the retroperitoneum was the metastasis from primary testicular tumor. In addition, marked hypoplasia of hematopoietic cells in the bone marrow, squamous metaplasia in the prostate and the gynecomastia were observed. It was suggested that the toxicity of hormones, such as estrogen and others, produced from the functional malignant Sertoli cell tumors was responsible for these lesions.
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6. クロミンククジラ(Balaenoptera bonaerensis)の葉状骨形成過程におけるVI型コラーゲンの発現に関する研究(第19回 日本獣医畜産大学学術交流会(動物医療センター竣工記念))日本獣医畜産大学研究報告 53 75-76 2004年12月1日
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日本獣医畜産大学研究報告 53 76-76 2004年12月1日
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Jpn. J. Environ. Toxicol. 6, 11-19 2003年
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日本獣医畜産大学研究報告 50 34-37 2001年12月ビテロジェニン（卵黄蛋白質前駆物質）についてメダカOryzias latipesで免疫組織化学的検索を行った。雌メダカにおいて肝臓と卵巣で陽性物質を認めたが、雄メダカでは陽性反応が認められなかった。このことから、メダカにおいてビテロジェニンを用いた免疫組織化学的手法が内分泌撹乱物質の検索に有用であることが示唆された。
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Bulletin of Nippon Veterinary and Animal Science University 50, 34-37 2001年
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Bulletin of Nippon Veterianry and Animal Science University 49, 1-4 2000年
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VETERINARY PATHOLOGY 37(1) 104-107 2000年1月Histopathologic and immunohistochemical examinations were performed on localized amyloidosis associated with mammary tumors in two dogs. These tumors were identified as adenoma and adenocarcinoma. An acellular, amorphous pale eosinophilic material (amyloid) was observed in the lumina of acini lined by neoplastic cells and in the stroma of the tumors. Concentrically laminated pale eosinophilic bodies (corpora amylacea) were also found in the lumina of the acini. Amyloid and corpora amylacea stained positively with Congo red with and without 5% potassium permanganate pretreatment and revealed a green birefringence under polarized light. Corpora amylacea showed an occasional Maltese-cross pattern. Immunohistochemically, amyloid and corpora amylacea usually stained positively with anti-bovine alpha-casein antibody but negatively with anti-human amyloid AA, anti-bovine kappa-light and lambda-light chains, anti-human lactoferrin, anti-human transferrin, anti human secretory component, and anti-human polyglucosan antibodies. These findings suggested that the amyloid deposition in these canine mammary tumors was related to lactating casein.
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Morphological changes of female reproductive organs of Japanese monkeys with reproductive conditionsPRIMATES 38(4) 359-367 1997年10月Macroscopic and histological changes were examined on the female reproductive organs of Japanese monkeys (Macaca fuscata) with varying reproductive status in enclosed and provisioned troops. The weight and size of reproductive organs are described. The size of uterus declined in 2-3 months after parturition. The number of parturition was roughly estimated by degrees of sclerosis in myometrial vessels. The nipple length of parous animals was longer than that of nulliparous ones. The bilateral difference in the length (more than 3 mm) between nipples indicated the experience of nursing. Interpreting ovaries on the basis of macroscopic examination alone was less precise. The present results suggested that the female sexual maturity and the experience of nursing can be estimated by the measurement of reproductive organs in the Japanese monkey.
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MARINE MAMMAL SCIENCE 13(2) 326-332 1997年4月
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JOURNAL OF FISH DISEASES 19(4) 271-277 1996年7月A large mass protruding beyond the left operculum was found in an adult male medaka, Oryzias latipes (Temminck & Schlegel). Histologically, the mass was mainly composed of thyroid follicles measuring up to 292 mu m in diameter. The follicular epithelium consisted of cuboidal or squamous cells. The follicles lacked colloidal material in the lumen. The thyroid tissue infiltrated into the kidney. Electron microscopically, the tumour cells were characterized by dilation and vesiculation of rough endoplasmic reticulum, which extended throughout the cytoplasm, and an irregular outline of the nucleus. Mitotic figures and partly degenerate cells were frequently observed. Based on the above features, the tumour was diagnosed as a follicular carcinoma of the thyroid, the first reported case in the medaka.
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Bulletin of Nippon Veterinary and Animal Science University 45 1-5 1996年
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ACTA NEUROPATHOLOGICA 88(1) 75-77 1994年7月Polyglucosan bodies (PGBs) in the brain of a 12-year-old Holstein cow exhibiting no signs of neurological abormality were examined by light and electron microscopy and immunohistochemistry. PGBs were disseminated throughout the brain, especially in the pallidum, thalamus and cerebellum. Cow PGBs were found in the neuronal perikaryon and in the neuropil. These were round, slightly to severely basophilic, and were strongly positive for the periodic acid-Schiff reaction. The cow PGBs were immunoreactive for monoclonal antibodies raised against human polyglucosan. Electron microscopic analysis revealed that they were composed of branching filaments, glycogen granules and electron-dense material. These findings indicate that cow PGBs closely resemble human or canine PGBs in Lafora's disease and PGBs found in aged animals that have been reported previously.
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Bulletin of Nippon Veterinary and Animal Science University 43 37-41 1994年
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JOURNAL OF INVERTEBRATE PATHOLOGY 62(3) 257-261 1993年11月
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PRIMATES 33(1) 133-137 1992年1月The present study examined the histochemistry of pigments in the corpus luteum of the ovaries of cynomolgus monkeys (Macaca fascicularis), Japanese monkeys (Macaca fuscata), and rhesus monkeys (Macaca mulatta). Yellowish brown pigments were found in the regressing corpus luteum cells. Histochemical studies revealed that these pigments consisted of lipofuscin, the so-called age pigment. The findings obtained suggest that accumulation of lipofuscin might be related to cellular aging of the corpus luteum.
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JOURNAL OF COMPARATIVE PATHOLOGY 105(4) 467-470 1991年11月
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JOURNAL OF COMPARATIVE PATHOLOGY 105(2) 241-245 1991年8月
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JOURNAL OF COMPARATIVE PATHOLOGY 104(2) 141-145 1991年2月
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HISTOLOGY AND HISTOPATHOLOGY 6(1) 21-24 1991年The brain from a Siamese cat with sphingomyelinosis was examined with lectin histochemistry. Swollen neurons were stained with Canavalia ensiformis agglutinin (Con A). Some of them were also stained with Ricinus communis agglutinin-I (RCA-I) and Ulex europaeus agglutinin-I (UEA-I). A small number of axonal spheroids and glia cells were positive for Con A, RCA-I, UEA-I and wheat germ agglutinin. Control tissues were weakly stained with Con A, but not with any of the other lectins. These results indicate that affected neurons contain mannose and glucose residues in addition to sphingomyelin. This study points to the possibility that the characteristics of lectin histochemical study might be helpful for the diagnosis of sphingomyelinosis.
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ACTA NEUROPATHOLOGICA 81(2) 217-218 1990年With the use of monoclonal antibodies, raised against the human polyglucosan, positive staining of polyglucosan bodies (PGB) was detected in the brain, spinal cord and cecum of aged dogs. PGB in feline brain were also positively stained with these antibodies. These findings indicate that animal PGB share common antigenicity with human PGB.
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JAPANESE JOURNAL OF VETERINARY SCIENCE 51(6) 1179-1184 1989年12月
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JOURNAL OF COMPARATIVE PATHOLOGY 101(3) 263-267 1989年10月
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ACTA ANATOMICA 136(1) 76-78 1989年9月
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日本獣医学雑誌 50(5) 1055-1059 1988年10月15日ウシの子宮動脈壁におけるGAGについて組織化学的に検索した. 動脈壁GAGは内膜および中膜にみられ,妊娠性硬変の進展とともに内膜肥厚部および中膜内層で増加する傾向が観察された. 酵素消化試験により, 増加するGAGがsulfated GAGであることが明らかとなったことから, ウシの子宮動脈にみられる妊娠性硬変の進展にはGAG, とくにsulfated GAGが密接に関与することが推察された.
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ANIMAL REPRODUCTION SCIENCE 16(3-4) 191-198 1988年8月
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日本獸醫學雜誌 43(4) 553-555 1981年8月25日昭和54年度特別天然記念物ニホンカモシカの保護管理に関する調査研究(文化庁)の一環として, 野生のメスニホンカモシカの性成熟, 妊娠および胎仔の発育を検討し, 約2.5年で性成熟に達するものと推定された. 成メス31例中26例(83.9%)は妊娠しており, 胎仔数はすべて1仔であった. 胎仔体長(CRL)の月別分布から胎仔の発育曲線を得, 図に示した.