研究者業績

神谷 新司

カミヤ シンジ  (Shinji Kamiya)

基本情報

所属
日本獣医生命科学大学 獣医学部 獣医保健看護学科 応用部門 教授
学位
the degree of doctor of veterinary science(Nippon Veterinary and Life Science University)
the degree of Master of veterinary science(Gifu University)

J-GLOBAL ID
200901011389859990
researchmap会員ID
1000093587

研究キーワード

 6

論文

 16
  • Hisashi Yoshimura, Kazushi Torikai, Anna Takahashi, Masaki Michishita, Takuya E Kishimoto, Masami Yamamoto, Makoto Haritani, Kimimasa Takahashi, Shinji Kamiya
    Journal of Comparative Pathology 201 28-32 2023年2月  
  • Hisashi Yoshimura, Maiko Moriya, Ayaka Yoshida, Masami Yamamoto, Yukino Machida, Kazuhiko Ochiai, Masaki Michishita, Takayuki Nakagawa, Yoko Matsuda, Kimimasa Takahashi, Shinji Kamiya, Toshiyuki Ishiwata
    Veterinary Pathology 030098582110186-030098582110186 2021年5月31日  
    Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.
  • Ayaka HATA, Yuto SUDA, Midori SAEKI, Tatsuki SHIMAMOTO, Hisashi YOSHIMURA, Masami YAMAMOTO, Aki FUJIWARA, Shinji KAMIYA, Makoto HARITANI
    Japanese Journal of Zoo and Wildlife Medicine 25(4) 141-145 2020年12月24日  
  • 岸本 拓也, 伊藤 優冴, 吉村 久志, 山本 昌美, 伊藤 圭子, 青木 博史, 常盤 俊大, 森田 達志, 播谷 亮, 神谷 新司
    日本獣医学会学術集会講演要旨集 163回 193-193 2020年10月  
  • H Yoshimura, A Otsuka, M Michishita, M Yamamoto, M Ashizawa, M Zushi, M Moriya, D Azakami, Y Matsuda, T Ishiwata, S Kamiya, K Takahashi
    Veterinary Pathology 56(3) 389-398 2019年3月  査読有り

MISC

 43
  • 銀梓, 藤平篤志, 三浦亮太朗, 水谷尚, 天尾弘実, 山田裕, 神谷新司, 吉村格, 望月眞理子
    日本獣医学会学術集会講演要旨集 160th 413 2017年8月30日  
  • 土屋萌, 落合和彦, 鈴木浩悦, 神谷新司, 加藤卓也, 名切幸枝, 石井奈穂美, 近江俊徳, 羽山伸一, 中西せつ子, 今野文治, 川本芳
    第33回日本霊長類学会大会, 福島市 2017年7月  
  • 若林真由, 銀梓, 佐藤巧, 藤平篤志, 三浦亮太朗, 水谷尚, 天尾弘実, 山田裕, 神谷新司, 吉村格, 望月眞理子
    動物介在教育・療法学雑誌(Web) 8(1-2) 17 (WEB ONLY) 2017年3月31日  
  • 山本 昌美, 吉村 久志, 木村 彩友子, 市野瀬 香鈴, 神谷 新司
    日本獣医生命科学大学研究報告 (65) 34-38 2016年12月1日  
    A male, mixed breed, over 8 years old dog had a mass palpated in the right cryptochidism located at the inguinal subcutaneous region. At necropsy another large mass was observed in the retroperitoneum. The masses in the right testis and in the retroperitoneum were approximately 7.5 cm and 10.0 cm in diameter, respectively. Histopathological examination revealed that both masses were composed of polygonal cells with moderate to high grade atypia. The tumor cells formed alveolar or cord structures separated by fibrous stroma, and showed a tendency to orient perpendicularly to the basement membrane. Immunohistochemically, the tumor cells were positive for vimentin, inhibin-α and WT1. The testis was diagnosed with malignant Sertoli cell tumor, and the mass in the retroperitoneum was the metastasis from primary testicular tumor. In addition, marked hypoplasia of hematopoietic cells in the bone marrow, squamous metaplasia in the prostate and the gynecomastia were observed. It was suggested that the toxicity of hormones, such as estrogen and others, produced from the functional malignant Sertoli cell tumors was responsible for these lesions.
  • 長田雅宏, 松原孝子, 藤澤正彦, 青木博史, 左向敏紀, 神谷新司, 吉村格, 望月眞理子
    関東畜産学会報 65(2) 45-50 2015年3月  

講演・口頭発表等

 1