Curriculum Vitaes

Shinji Kamiya

  (神谷 新司)

Profile Information

Affiliation
Professor, Nippon Veterinary and Life Science University
Degree
the degree of doctor of veterinary science(Nippon Veterinary and Life Science University)
the degree of Master of veterinary science(Gifu University)

J-GLOBAL ID
200901011389859990
researchmap Member ID
1000093587

Research Interests

 6

Papers

 16
  • Hisashi Yoshimura, Kazushi Torikai, Anna Takahashi, Masaki Michishita, Takuya E Kishimoto, Masami Yamamoto, Makoto Haritani, Kimimasa Takahashi, Shinji Kamiya
    Journal of Comparative Pathology, 201 28-32, Feb, 2023  
  • Hisashi Yoshimura, Maiko Moriya, Ayaka Yoshida, Masami Yamamoto, Yukino Machida, Kazuhiko Ochiai, Masaki Michishita, Takayuki Nakagawa, Yoko Matsuda, Kimimasa Takahashi, Shinji Kamiya, Toshiyuki Ishiwata
    Veterinary Pathology, 030098582110186-030098582110186, May 31, 2021  
    Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.
  • Ayaka HATA, Yuto SUDA, Midori SAEKI, Tatsuki SHIMAMOTO, Hisashi YOSHIMURA, Masami YAMAMOTO, Aki FUJIWARA, Shinji KAMIYA, Makoto HARITANI
    Japanese Journal of Zoo and Wildlife Medicine, 25(4) 141-145, Dec 24, 2020  
  • 岸本 拓也, 伊藤 優冴, 吉村 久志, 山本 昌美, 伊藤 圭子, 青木 博史, 常盤 俊大, 森田 達志, 播谷 亮, 神谷 新司
    日本獣医学会学術集会講演要旨集, 163回 193-193, Oct, 2020  
  • H Yoshimura, A Otsuka, M Michishita, M Yamamoto, M Ashizawa, M Zushi, M Moriya, D Azakami, Y Matsuda, T Ishiwata, S Kamiya, K Takahashi
    Veterinary Pathology, 56(3) 389-398, Mar, 2019  Peer-reviewed

Misc.

 43

Presentations

 1