Profile Information
- Affiliation
- Professor, Nippon Veterinary and Life Science University
- Degree
- the degree of doctor of veterinary science(Nippon Veterinary and Life Science University)the degree of Master of veterinary science(Gifu University)
- J-GLOBAL ID
- 200901011389859990
- researchmap Member ID
- 1000093587
Research Areas
3Research History
8-
Apr, 2011 - Present
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Apr, 1996 - Mar, 2011
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1988 - 1996
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1988 - 1996
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1980 - 1988
Education
4Papers
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Journal of Comparative Pathology, 201 28-32, Feb, 2023
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Veterinary Pathology, 030098582110186-030098582110186, May 31, 2021Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.
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Severe Jackknife-like Kyphosis Malformation in the Fetus of a Free-ranging Sika Deer (Cervus nippon)Japanese Journal of Zoo and Wildlife Medicine, 25(4) 141-145, Dec 24, 2020
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Veterinary Pathology, 56(3) 389-398, Mar, 2019 Peer-reviewed
Misc.
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(65) 34-38, Dec 1, 2016
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関東畜産学会報, 65(2) 45-50, Mar, 2015
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6. クロミンククジラ(Balaenoptera bonaerensis)の葉状骨形成過程におけるVI型コラーゲンの発現に関する研究(第19回 日本獣医畜産大学学術交流会(動物医療センター竣工記念))日本獣医畜産大学研究報告, 53 75-76, Dec 1, 2004
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日本獣医畜産大学研究報告, 53 76-76, Dec 1, 2004
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Jpn. J. Environ. Toxicol., 6, 11-19, 2003
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The bulletin of the Nippon Veterinary and Zootechnical College, 50 34-37, Dec, 2001
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Bulletin of Nippon Veterinary and Animal Science University, 50, 34-37, 2001
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Bulletin of Nippon Veterianry and Animal Science University, 49, 1-4, 2000
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VETERINARY PATHOLOGY, 37(1) 104-107, Jan, 2000Histopathologic and immunohistochemical examinations were performed on localized amyloidosis associated with mammary tumors in two dogs. These tumors were identified as adenoma and adenocarcinoma. An acellular, amorphous pale eosinophilic material (amyloid) was observed in the lumina of acini lined by neoplastic cells and in the stroma of the tumors. Concentrically laminated pale eosinophilic bodies (corpora amylacea) were also found in the lumina of the acini. Amyloid and corpora amylacea stained positively with Congo red with and without 5% potassium permanganate pretreatment and revealed a green birefringence under polarized light. Corpora amylacea showed an occasional Maltese-cross pattern. Immunohistochemically, amyloid and corpora amylacea usually stained positively with anti-bovine alpha-casein antibody but negatively with anti-human amyloid AA, anti-bovine kappa-light and lambda-light chains, anti-human lactoferrin, anti-human transferrin, anti human secretory component, and anti-human polyglucosan antibodies. These findings suggested that the amyloid deposition in these canine mammary tumors was related to lactating casein.
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Morphological changes of female reproductive organs of Japanese monkeys with reproductive conditionsPRIMATES, 38(4) 359-367, Oct, 1997Macroscopic and histological changes were examined on the female reproductive organs of Japanese monkeys (Macaca fuscata) with varying reproductive status in enclosed and provisioned troops. The weight and size of reproductive organs are described. The size of uterus declined in 2-3 months after parturition. The number of parturition was roughly estimated by degrees of sclerosis in myometrial vessels. The nipple length of parous animals was longer than that of nulliparous ones. The bilateral difference in the length (more than 3 mm) between nipples indicated the experience of nursing. Interpreting ovaries on the basis of macroscopic examination alone was less precise. The present results suggested that the female sexual maturity and the experience of nursing can be estimated by the measurement of reproductive organs in the Japanese monkey.
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MARINE MAMMAL SCIENCE, 13(2) 326-332, Apr, 1997
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JOURNAL OF FISH DISEASES, 19(4) 271-277, Jul, 1996A large mass protruding beyond the left operculum was found in an adult male medaka, Oryzias latipes (Temminck & Schlegel). Histologically, the mass was mainly composed of thyroid follicles measuring up to 292 mu m in diameter. The follicular epithelium consisted of cuboidal or squamous cells. The follicles lacked colloidal material in the lumen. The thyroid tissue infiltrated into the kidney. Electron microscopically, the tumour cells were characterized by dilation and vesiculation of rough endoplasmic reticulum, which extended throughout the cytoplasm, and an irregular outline of the nucleus. Mitotic figures and partly degenerate cells were frequently observed. Based on the above features, the tumour was diagnosed as a follicular carcinoma of the thyroid, the first reported case in the medaka.
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Bulletin of Nippon Veterinary and Animal Science University, 45 1-5, 1996
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Bulletin of Nippon Veterinary and Animal Science University, 44 1-4, 1995
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ACTA NEUROPATHOLOGICA, 88(1) 75-77, Jul, 1994Polyglucosan bodies (PGBs) in the brain of a 12-year-old Holstein cow exhibiting no signs of neurological abormality were examined by light and electron microscopy and immunohistochemistry. PGBs were disseminated throughout the brain, especially in the pallidum, thalamus and cerebellum. Cow PGBs were found in the neuronal perikaryon and in the neuropil. These were round, slightly to severely basophilic, and were strongly positive for the periodic acid-Schiff reaction. The cow PGBs were immunoreactive for monoclonal antibodies raised against human polyglucosan. Electron microscopic analysis revealed that they were composed of branching filaments, glycogen granules and electron-dense material. These findings indicate that cow PGBs closely resemble human or canine PGBs in Lafora's disease and PGBs found in aged animals that have been reported previously.
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Bulletin of Nippon Veterinary and Animal Science University, 43 37-41, 1994
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JOURNAL OF INVERTEBRATE PATHOLOGY, 62(3) 257-261, Nov, 1993
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PRIMATES, 33(1) 133-137, Jan, 1992The present study examined the histochemistry of pigments in the corpus luteum of the ovaries of cynomolgus monkeys (Macaca fascicularis), Japanese monkeys (Macaca fuscata), and rhesus monkeys (Macaca mulatta). Yellowish brown pigments were found in the regressing corpus luteum cells. Histochemical studies revealed that these pigments consisted of lipofuscin, the so-called age pigment. The findings obtained suggest that accumulation of lipofuscin might be related to cellular aging of the corpus luteum.
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JOURNAL OF COMPARATIVE PATHOLOGY, 105(4) 467-470, Nov, 1991
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JOURNAL OF COMPARATIVE PATHOLOGY, 105(2) 241-245, Aug, 1991
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JOURNAL OF COMPARATIVE PATHOLOGY, 104(2) 141-145, Feb, 1991
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HISTOLOGY AND HISTOPATHOLOGY, 6(1) 21-24, 1991The brain from a Siamese cat with sphingomyelinosis was examined with lectin histochemistry. Swollen neurons were stained with Canavalia ensiformis agglutinin (Con A). Some of them were also stained with Ricinus communis agglutinin-I (RCA-I) and Ulex europaeus agglutinin-I (UEA-I). A small number of axonal spheroids and glia cells were positive for Con A, RCA-I, UEA-I and wheat germ agglutinin. Control tissues were weakly stained with Con A, but not with any of the other lectins. These results indicate that affected neurons contain mannose and glucose residues in addition to sphingomyelin. This study points to the possibility that the characteristics of lectin histochemical study might be helpful for the diagnosis of sphingomyelinosis.
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ACTA NEUROPATHOLOGICA, 81(2) 217-218, 1990With the use of monoclonal antibodies, raised against the human polyglucosan, positive staining of polyglucosan bodies (PGB) was detected in the brain, spinal cord and cecum of aged dogs. PGB in feline brain were also positively stained with these antibodies. These findings indicate that animal PGB share common antigenicity with human PGB.
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JAPANESE JOURNAL OF VETERINARY SCIENCE, 51(6) 1179-1184, Dec, 1989
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JOURNAL OF COMPARATIVE PATHOLOGY, 101(3) 263-267, Oct, 1989
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ACTA ANATOMICA, 136(1) 76-78, Sep, 1989
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Japanese journal of veterinary science, 50(5) 1055-1059, Oct 15, 1988The relationship between glycosaminoglycans (GAG) and the pregnancy-induced sclerosis of the bovine uterine artery was histochemically studied. Both sulfated and non-sulfated GAG were observed in the arteries. As advancing the intimal thickening and elastosis, GAG seemed to increase in amount in the thickened intima and the inner half of the media. Digestion with Streptomyces hyaluronidase slightly altered the alcian blue stainability of the uterine arteries, whereas digestion with chondroitinase ABC apparently reduced the alcianophilia of the arteries. It is, therefore, assumed that the sulfated GAG such as chondroitin sulfates might correlate with advancing the pregnancy-induced sclerosis.
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ANIMAL REPRODUCTION SCIENCE, 16(3-4) 191-198, Aug, 1988
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The bulletin of the Nippon Veterinary and Zootechnical College, 33 23-27, 1984
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The bulletin of the Nippon Veterinary and Zootechnical College, 32 1-5, 1983
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The bulletin of the Nippon Veterinary and Zootechnical College, 32 38-47, 1983
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The Japanese journal of veterinary science, 43(4) 553-555, Aug 25, 1981