CVClient

Daisuke Yamauchi

  (山内 大輔)

Profile Information

Affiliation
Associate Professor, University of Hyogo
Degree
D.Sc.(Tokyo Metropolitan University)
M.Sc.(Tokyo Metropolitan University)

J-GLOBAL ID
200901080373900437
researchmap Member ID
1000053925

Committee Memberships

 3

Major Papers

 39
  • YAMAUCHI Daisuke, Aki Fukuda, Ichiro Karahara, Mineyuki Yoshinobu
    Plant Morphology, 28 3-7, 2016  Peer-reviewedInvited
  • Keita Sutoh, Kenji Washio, Ryozo Imai, Masamitsu Wada, Tomonori Nakai, Daisuke Yamauchi
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(5) 747-759, May, 2015  Peer-reviewed
    The expression of the gene for a proteinase (Rep1) is upregulated by gibberellins. The CAACTC regulatory element (CARE) of the Rep1 promoter is involved in the gibberellin response. We isolated a cDNA for a CARE-binding protein containing a Myb domain in its carboxyl-terminal region and designated the gene Carboxyl-terminal Myb1 (CTMyb1). This gene encodes two polypeptides of two distinctive lengths, CTMyb1L and CTMyb1S, which include or exclude 213N-terminal amino acid residues, respectively. CTMyb1S transactivated the Rep1 promoter in the presence of OsGAMyb, but not CTMyb1L. We observed an interaction between CTMyb1S and the rice prolamin box-binding factor (RPBF). A bimolecular fluorescence complex analysis detected the CTMyb1S and RPBF complex in the nucleus, but not the CTMyb1L and RPBF complex. The results suggest that the arrangement of the transfactors is involved in gibberellin-inducible expression of Rep1.
  • Daisuke Yamauchi, Daisuke Tamaoki, Masato Hayami, Miyuki Takeuchi, Ichirou Karahara, Mayuko Sato, Kiminori Toyooka, Hiroshi Nishioka, Yasuko Terada, Kentaro Uesugi, Hidekazu Takano, Yasushi Kagoshima, Yoshinobu Mineyuki
    Journal of Electron Microscopy, 62(3) 353-361, Jun, 2013  Peer-reviewed
    The cotyledon of legume seeds is a storage organ that provides nutrients for seed germination and seedling growth. The spatial and temporal control of the degradation processes within cotyledons has not been elucidated. Calcium oxalate (CaOx) crystals, a common calcium deposit in plants, have often been reported to be present in legume seeds. In this study, micro-computed tomography (micro-CT) was employed at the SPring-8 facility to examine the three-dimensional distribution of crystals inside cotyledons during seed maturation and germination of Lotus miyakojimae (previously Lotus japonicus accession Miyakojima MG-20). Using this technique, we could detect the outline of the embryo, void spaces in seeds and the cotyledon venation pattern. We found several sites that strongly inhibited X-ray transmission within the cotyledons. Light and polarizing microscopy confirmed that these areas corresponded to CaOx crystals. Three-dimensional observations of dry seeds indicated that the CaOx crystals in the L. miyakojimae cotyledons were distributed along lateral veins however, their distribution was limited to the abaxial side of the procambium. The CaOx crystals appeared at stage II (seed-filling stage) of seed development, and their number increased in dry seeds. The number of crystals in cotyledons was high during germination, suggesting that CaOx crystals are not degraded for their calcium supply. Evidence for the conservation of CaOx crystals in cotyledons during the L. miyakojimae germination process was also supported by the biochemical measurement of oxalic acid levels. © The Author 2012. Published by Oxford University Press [on behalf of Japanese Society of Microscopy]. All rights reserved.

Misc.

 72

Books and Other Publications

 1

Presentations

 5

Teaching Experience

 4

Research Projects

 11