Faculty of Veterinary Science
基本情報
- 所属
- 日本獣医生命科学大学 獣医学部 獣医保健看護学科 応用部門 教授
- 学位
- the degree of doctor of veterinary science(Nippon Veterinary and Life Science University)the degree of Master of veterinary science(Gifu University)
- J-GLOBAL ID
- 200901011389859990
- researchmap会員ID
- 1000093587
経歴
8-
2011年4月 - 現在
-
1996年4月 - 2011年3月
-
1988年 - 1996年
-
1988年 - 1996年
-
1980年 - 1988年
学歴
4-
- 1980年
-
- 1980年
-
- 1978年
-
- 1978年
論文
16-
Journal of Comparative Pathology 201 28-32 2023年2月
-
Veterinary Pathology 030098582110186-030098582110186 2021年5月31日Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.
-
Severe Jackknife-like Kyphosis Malformation in the Fetus of a Free-ranging Sika Deer (Cervus nippon)Japanese Journal of Zoo and Wildlife Medicine 25(4) 141-145 2020年12月24日
-
Veterinary Pathology 56(3) 389-398 2019年3月 査読有り
MISC
43-
日本獣医生命科学大学研究報告 (65) 34-38 2016年12月1日A male, mixed breed, over 8 years old dog had a mass palpated in the right cryptochidism located at the inguinal subcutaneous region. At necropsy another large mass was observed in the retroperitoneum. The masses in the right testis and in the retroperitoneum were approximately 7.5 cm and 10.0 cm in diameter, respectively. Histopathological examination revealed that both masses were composed of polygonal cells with moderate to high grade atypia. The tumor cells formed alveolar or cord structures separated by fibrous stroma, and showed a tendency to orient perpendicularly to the basement membrane. Immunohistochemically, the tumor cells were positive for vimentin, inhibin-α and WT1. The testis was diagnosed with malignant Sertoli cell tumor, and the mass in the retroperitoneum was the metastasis from primary testicular tumor. In addition, marked hypoplasia of hematopoietic cells in the bone marrow, squamous metaplasia in the prostate and the gynecomastia were observed. It was suggested that the toxicity of hormones, such as estrogen and others, produced from the functional malignant Sertoli cell tumors was responsible for these lesions.